Saharuddin bin Mohamad received his Bachelor of Engineering (Bioengineering) in 1998 and Master of Engineering (Bioengineering) in 2000 from Tokushima University, Japan. He subsequently obtained his Doctor of Engineering in Functional Systems Engineering from the same university in 2003. Following his doctoral studies, he undertook postdoctoral research in bioinformatics at the Nara Institute of Science and Technology (NAIST), Japan, until 2004.

He joined Universiti Malaya in 2004 as a lecturer in the Bioinformatics Programme at the Institute of Biological Sciences, Faculty of Science, and has since been actively involved in teaching, curriculum development, research, and academic leadership. He has contributed extensively to the Bachelor of Science in Bioinformatics and Master of Bioinformatics programmes and has held several leadership positions within Universiti Malaya, including Programme Coordinator of the Bachelor of Science in Bioinformatics programme and Head of the Institute of Biological Sciences.

He is a bioinformatician with expertise spanning structural bioinformatics, molecular bioinformatics, translational bioinformatics, and artificial intelligence applications in biological, biomedical, and clinical research. His work in structural bioinformatics focuses on computer-aided drug design, molecular docking, molecular dynamics simulations, and protein engineering. In molecular bioinformatics, his research encompasses next-generation sequencing (NGS) data analysis, including genomics, metagenomics, whole-exome sequencing, and transcriptomics. His translational bioinformatics research integrates genomic and clinical data to support disease-variant interpretation, rare-disease diagnosis, biomarker discovery, and precision medicine. More recently, his research has increasingly incorporated artificial intelligence, machine learning, and data mining for the analysis of complex biological and multi-omics data and their translation into biomedical and clinical applications.

He has also contributed actively to the bioinformatics community, serving as Vice President of the Malaysian Society of Bioinformatics and Computational Biology (MaSBiC) for two consecutive terms from 2018 to 2022 and as an Advisory Board Member of MyBioInfoNet from 2019 to 2021.

Currently, he leads the Centre of Research in Systems Biology, Structural Bioinformatics and Human Digital Imaging (CRYSTAL), where he oversees research in structural bioinformatics, AI-driven biomedical data analysis, and translational bioinformatics, while fostering interdisciplinary collaborations in precision medicine and computational biology.