Saharuddin bin Mohamad received his Bachelor of Engineering (Bioengineering) in 1998 and Master of Engineering (Bioengineering) in 2000 from Tokushima University, Japan. He subsequently obtained his Doctor of Engineering degree in Functional Systems Engineering from the same university in 2003. Following his doctoral studies, he undertook a postdoctoral position in bioinformatics at the Nara Institute of Science and Technology (NAIST), Japan, until 2004.

He joined Universiti Malaya in 2004 as a lecturer in the Bioinformatics Programme at the Institute of Biological Sciences, Faculty of Science. and has since served in numerous academic leadership roles. He has been actively involved in teaching and curriculum development for the Bachelor of Science in Bioinformatics and Master of Bioinformatics programmes at Universiti Malaya. He has held several leadership positions within Universiti Malaya, including Programme Coordinator of the Bachelor of Science in Bioinformatics programme and Head of the Institute of Biological Sciences.

He is a bioinformatician whose expertise spans structural bioinformatics, molecular bioinformatics, translational bioinformatics, and artificial intelligence for biotechnology, biomedical, and clinical data analysis. His work in structural bioinformatics focuses on computer-aided drug design, molecular docking, molecular dynamics simulations, and protein engineering. In molecular bioinformatics, he specialises in the analysis of next-generation sequencing (NGS) data, including genomics, metagenomics, whole-exome sequencing, and transcriptomics. His translational bioinformatics research integrates genomic and clinical data to support disease variant interpretation, rare disease diagnosis, biomarker discovery, and precision medicine. More recently, he has incorporated artificial intelligence (AI), machine learning, and data mining to analyse complex multi-omics datasets and accelerate biological discovery and clinical translation.

He has also contributed actively to the bioinformatics community through his service as Vice President of the Malaysian Society of Bioinformatics and Computational Biology (MaSBiC) for two consecutive terms (2018–2022) and as an Advisory Board Member of MyBioInfoNet (2019–2021).

He currently leads the Centre of Research in Systems Biology, Structural Bioinformatics and Human Digital Imaging (CRYSTAL), where he spearheads research in structural bioinformatics, AI-driven biomedical data analysis, and translational bioinformatics while fostering interdisciplinary collaborations in precision medicine and computational biology.