Curriculum Vitae

DR. MOHD ISKANDAR JEFREE BIN JOHARI

Research Officer
  • Tropical Infectious Diseases Research and Education Centre
  • jefree
  • 0379676670

AREAS OF EXPERTISE

  • EPIDEMIOLOGY
    INFECTIOUS DISEASES
  • VIROLOGY
    RESPIRATORY VIRUSES & ARBOVIRUSES
  • MEDICAL BIOTECHNOLOGY
    MOLECULAR AND SEROLOGY ASSAY

ACADEMIC QUALIFICATION

  • BSc, (MICROBIOLOGY & IMMUNOLOGY)
    University of Otago
  • PhD, (INFECTIOUS DISEASE EPIDEMIOLOGY)
    Universiti Malaya (UM)
  • MMedSc, (MOLECULAR BIOLOGY)
    Universiti Malaya (UM)

MEMBERSHIPS

  • MALAYSIA BOARD OF TECHNOLOGISTS (MBOT), GRADUATE MEMBER
    Since 2020 (National)
  • MALAYSIAN BIOSAFETY & BIOSECURITY ASSOCIATION (MBBA), LIFE MEMBER
    Since 2015 (National)
  • MALAYSIAN SOCIETY FOR MOLECULAR BIOLOGY & BIOTECHNOLOGY (MSMBB), ORDINARY MEMBER
    2019 to 2019 (National)
  • MALAYSIAN SOCIETY OF PARASITOLOGY & TROPICAL MEDICINE (MSPTM), ORDINARY MEMBER
    2016 to 2016 (National)

AWARD AND STEWARDSHIP

  • EXCELLENCE SERVICE AWARD
    University of Malaya, 2023 (University)
  • BEST PRESENTATION AWARD
    International Conference of UM Academia - Community Engagement, 2019 (International)
  • CERTIFICATE OF EXCELLENT SERVICE
    University of Malaya, 2015 (University)
  • Phlebotomy Certification
    Medicskills Sdn Bhd, 2015 (International)
  • Biosafety Level 2 (BSL2) & Biosafety Level 3 (BSL3) Training Certification
    Universiti Malaya, 2015 (University)
  • Responsible Care & Use of Laboratory Animals Course (RCULAC) Certification
    Universiti Malaya, 2015 (University)
  • Anugerah Inovasi Negara (Kategori Akar Umbi)
    Kementerian Sains, Teknologi, & Inovasi, 2015 (National)
  • Good Clinical Practice (GCP) Certification
    Kementerian Kesihatan, 2014 (National)

PUBLICATIONS

Article in Journal
WoS
  1. Johari, Jefree; Hontz, Robert. D. D.; Pike, Brian. L. L.; Husain, Tupur; Rusli, Norhayati; Mohd-Zain, Rozainanee; Tiong, Vunjia; Lee, Hai-Yen; Teoh, Boon-Teong; Sam, Sing-Sin; Khor, Chee-Sieng; Loong, Shih-Keng; Abd-Jamil, Juraina; Nor'e, Siti-Sarah; Yahaya, Hasmawati; Che-Kamaruddin, Naim; Garcia-Rivera, Jose. A. A.; AbuBakar, Sazaly (2023). MERS-CoV seroconversion amongst Malaysian Hajj pilgrims returning from the Middle East, 2016-2018: results from the MERCURIAL multiyear prospective cohort study, EMERGING MICROBES & INFECTIONS. 12(1). doi:10.1080/22221751.2023.2208678
  2. Johari, J., Hontz, R. D., Pike, B. L., Husain, T., Chong, C. K., Rusli, N., . . . AbuBakar, S. (2021). Multiyear prospective cohort study to evaluate the risk potential of MERS-CoV infection among Malaysian Hajj pilgrims (MERCURIAL): a study protocol. BMJ Open, 11(8), 6. doi: 10.1136/bmjopen-2021-050901
  3. Tan, K. K., Tiong, V., Tan, J. Y., Wong, J. E., Teoh, B. T., Abd-Jamil, J., . . . AbuBakar, S. (2021). Multiplex sequencing of SARS-Cov-2 genome directly from clinical samples using the Ion Personal Genome Machine (PGM). Tropical Biomedicine, 38(3), 283-288. doi: 10.47665/tb.38.3.069
  4. Tan, Kim-Kee; Tan, Jia-Yi; Wong, Jo-Ern; Teoh, Boon-Teong; Tiong, Vunjia; Abd-Jamil, Juraina; Nor'e, Siti-Sarah; Khor, Chee-Sieng; Johari, Jefree; Yaacob, Che-Norainon; Zulkifli, Mulya-Mustika-Sari; CheMatSeri, AsmaAnati; Mahfodz, Nur-Hidayana; Azizan, Noor Syahida; AbuBakar, Sazaly (2021). Emergence of B.1.524(G) SARS-CoV-2 in Malaysia during the third COVID-19 epidemic wave, SCIENTIFIC REPORTS. 11(1). doi:10.1038/s41598-021-01223-4
  5. Alghazali, K. A., Teoh, B. T., Sam, S. S., Abd-Jamil, J., Johari, J., Atroosh, W. M., . . . AbuBakar, S. (2020). Dengue fever among febrile patients in Taiz City, Yemen during the 2016 war: Clinical manifestations, risk factors, and patients knowledge, attitudes, and practices toward the disease. One Health, 9, 9. doi: 10.1016/j.onehlt.2019.100119
  6. Alghazali, KhairAlah A.; Teoh, Boon-Teong; Loong, Shih-Keng; Sam, Sing-Sin; Che-Mat-Seri, Nurul-Asma-Anati; Samsudin, Nur-Izyan; Yaacob, Che-Norainon; Azizan, Noor-Syahida; Oo, Adrian; Baharudin, Nur-Adilah; Tan, Kim-Kee; Abd-Jamil, Juraina; Nor'e, Siti-Sarah; Khor, Chee-Sieng; Johari, Jefree; Mahdy, Mohammed A. K.; AbuBakar, Sazaly (2019). Dengue Outbreak during Ongoing Civil War, Taiz, Yemen, EMERGING INFECTIOUS DISEASES. 25(7), 1397-1400. doi:10.3201/eid2507.180046
  7. Khor, Chee-Sieng; Hassan, Habibi; Mohd-Rahim, Nurul-Farhana; Chandren, Josephine Rebecca; Nore, Siti-Sarah; Johari, Jefree; Loong, Shih-Keng; Abd-Jamil, Juraina; Khoo, Jing-Jing; Lee, Hai-Yen; Pike, Brian L.; Ping, Wong Li; Lim, Yvonne Ai-Lian; Abubakar, Sazaly (2019). Seroprevalence of Borrelia burgdorferi among the indigenous people (Orang Asli) of Peninsular Malaysia, JOURNAL OF INFECTION IN DEVELOPING COUNTRIES. 13(5), 449-454. doi:10.3855/jidc.11001
  8. Khor, C. S., Mohd-Rahim, N. F., Hassan, H., Chandren, J. R., Nore, S. S., Johari, J., . . . AbuBakar, S. (2018). Seroprevalence of Q Fever Among the Indigenous People (Orang Asli) of Peninsular Malaysia. Vector-Borne and Zoonotic Diseases, 7. doi:10.1089/vbz.2017.2153
  9. Loong, Shih Keng; Che-Mat-Seri, Nurul-Asma-Anati; Abdulrazak, Osama; Douadi, Benacer; Ahmad-Nasrah, Siti-Noraisah; Johari, Jefree; Mohd-Zain, Siti-Nursheena; Abubakar, Sazaly (2018). Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia, JOURNAL OF VETERINARY MEDICAL SCIENCE. 80(1), 77-84. doi:10.1292/jvms.17-0218
  10. Zainal, Nurhafiza; Tan, Kim-Kee; Johari, Jefree; Hussein, Heselynn; Musa, Wan Rosmaiza Wan; Hassan, Jamiyah; Lin, Yee-Shin; AbuBakar, Sazaly (2018). Sera of patients with systemic lupus erythematosus cross-neutralizes dengue viruses, MICROBIOLOGY AND IMMUNOLOGY. 62(10), 659-672. doi:10.1111/1348-0421.12652
  11. Loong, S. K.; Johari, J.; Seri, Che Mat N. A. A.; AbdulRazak, O.; Douadi, B.; Nasrah, Ahmad S. N.; Zain, Mohd S. N.; AbuBakar, S. (2016). Isolation and Identification of an Emerging Pathogen, Kocuria marina, from Rattus rattus diardii, TROPICAL BIOMEDICINE. 33(3), 589-593
  12. Loong, Shih Keng; Soh, Yih Harng; Mahfodz, Nur Hidayana; Johari, Jefree; AbuBakar, Sazaly (2016). Synovial Tissue Infection with Burkholderia fungorum, EMERGING INFECTIOUS DISEASES. 22(10), 1834-1835. doi:10.3201/eid2210.151114
  13. Teoh, Boon-Teong; Sam, Sing-Sin; Tan, Kim-Kee; Johari, Jefree; Abd-Jamil, Juraina; Hooi, Poh-Sim; AbuBakar, Sazaly (2016). The Use of NS1 Rapid Diagnostic Test and qRT-PCR to Complement IgM ELISA for Improved Dengue Diagnosis from Single Specimen, SCIENTIFIC REPORTS. 6. doi:10.1038/srep27663
  14. AbuBakar, Sazaly; Teoh, Boon-Teong; Sam, Sing-Sin; Chang, Li-Yen; Johari, Jefree; Hooi, Poh-Sim; Lakhbeer-Singh, Harvinder-Kaur; Italiano, Claire M.; Omar, Sharifah F. Syed; Wong, Kum-Thong; Ramli, Norlisah; Tan, Chong-Tin (2013). Outbreak of Human Infection with Sarcocystis nesbitti, Malaysia, 2012, EMERGING INFECTIOUS DISEASES. 19(12), 1989-1991. doi:10.3201/eid1912.120530
  15. Teoh, Boon-Teong; Sam, Sing-Sin; Tan, Kim-Kee; Johari, Jefree; Danlami, Mohammed Bashar; Hooi, Poh-Sim; Md-Esa, Rafi; AbuBakar, Sazaly (2013). Detection of dengue viruses using reverse transcription-loop-mediated isothermal amplification, BMC INFECTIOUS DISEASES. 13. doi:10.1186/1471-2334-13-387
  16. Teoh, Boon-Teong; Sam, Sing-Sin; Tan, Kim-Kee; Johari, Jefree; Shu, Meng-Hooi; Danlami, Mohammed Bashar; Abd-Jamil, Juraina; MatRahim, NorAziyah; Mahadi, Nor Muhammad; AbuBakar, Sazaly (2013). Dengue virus type 1 clade replacement in recurring homotypic outbreaks, BMC EVOLUTIONARY BIOLOGY. 13. doi:10.1186/1471-2148-13-213
  17. Johari, Jefree; Kianmehr, Aynaz; Mustafa, Mohd Rais; Abubakar, Sazaly; Zandi, Keivan (2012). Antiviral Activity of Baicalein and Quercetin against the Japanese Encephalitis Virus, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 13(12), 16785-16795. doi:10.3390/ijms131216785
  18. Zandi, Keivan; Lani, Rafidah; Wong, Pooi-Fong; Teoh, Boon-Teong; Sam, Sing-Sin; Johari, Jefree; Mustafa, Mohd Rais; AbuBakar, Sazaly (2012). Flavone Enhances Dengue Virus Type-2 (NGC Strain) Infectivity and Replication in Vero Cells, MOLECULES. 17(3), 2437-2445. doi:10.3390/molecules17032437
Books
  1. Malaysia Laboratory Biosafety & Biosecurity: Policy & Guideline, First Edition (2015), Ministry of Health Malaysia
Chapter in Books
  1. UM Research (UMR) Bulletin, Volume 1 (2015), University of Malaya
Other Publications
  1. Johari, J. (2018). Penyakit MERS-CoV - Terbawa Pulang Tanpa Sengaja. Rancangan Ala Carte Pagi. Bernama Radio - Mass Media(Newspaper,Radio,Tv,Popular Magazine)

PAPER PRESENTED

INVITED SPEAKER
  1. Multi-Year Prospective Cohort Study to Evaluate the Risk Potential of MERS-CoV, 2016 - 2018, DTRA Chemical and Biological Defense Science & Technology Conference 2019, Defense Threat Reduction Agency (DTRA) (International) (18 Nov 2019 - 21 Nov 2019)
ORAL PRESENTER
  1. Multiyear Prospective Cohort Study to Evaluate The Risk Potential of MERS-CoV Infection Among Malaysian Hajj Pilgrims: 2016~2018, 1st Joint Meeting TIDCL/CRCIV/TIDREC/IMPE, Ajou University, Republic of Korea (International) (17 May 2022 - 17 May 2022)
  2. Weaponizing community in fight against dengue and dengue vector, International Conference of UM Academia - Community Engagement, UM Community & Sustainability Centre (UMCares) (International) (30 Sep 2019 - 01 Oct 2019)
POSTER PRESENTER
  1. Weaponizing community in fight against dengue and dengue vector, International Conference of UM Academia - Community Engagement, UM Community & Sustainability Centre (UMCares) (International) (30 Sep 2019 - 01 Oct 2019)
  2. Knowledge and technology transfer of an innovative Spokojan mosquito coil and holder system for outdoor deployment to Orang Asli community in Jelebu, Negeri Sembilan, Second National Conference on Knowledge Transfer (KTP 02) , KTP Secretariat @USM (National) (09 Sep 2014 - 11 Sep 2014)
  3. Dengue virus infection induces endothelial cells senescence, The 16th International Congress on Infectious Diseases, International Society for Infectious Diseases (International) (02 Apr 2014 - 05 Apr 2014)
  4. Reverse transcription-recombinase polymerase amplification assay for rapid detection of dengue virus, The 62nd Annual Meeting of the American Society of Tropical Medicine and Hygiene , The American Journal of Tropical Medicine and Hygiene (International) (13 Nov 2013 - 17 Nov 2013)
  5. Development of a simple single-tube reverse transcription-loop-mediated isothermal amplification assay for detection of dengue virus from clinical samples, The 8th European Congress on Tropical Medicine and International Health , The Federation of European Societies for Tropical Medicine and International Health (International) (10 Sep 2013 - 13 Sep 2013)
  6. Comparison of real time reverse transcription-polymerase chain reaction assays for the detection of Dengue virus. , Singapore International Conference on Dengue and Emerging Infections , STOP Dengue Translational Clinical Research Program and the Duke NUS Emerging Infectious Diseases Program (International) (21 Nov 2012 - 23 Nov 2012)
  7. Purifying selection in the evolution of Dengue virus type 1 in Malaysia, The 15th International Congress on Infectious Diseases, International Society for Infectious Diseases (International) (13 Jun 2012 - 16 Jun 2012)
  8. Phylogenetic analysis of Dengue virus type 1 non-structural-5 gene in recurring Dengue outbreaks in Malaysia. , Colloquium on Updates on Dengue and Arbovirus Research in Malaysia, Tropical Infectious Diseases Research & Education Centre (TIDREC) (National) (12 Apr 2010 - 13 Apr 2010)
  9. Phylogenetic analysis of Dengue virus type 1 non-structural-1 gene in recurring dengue outbreaks in Malaysia., Duke-NUS Emerging Infectious Disease Inauguration Symposium, Duke-NUS Graduate Medical School (International) (08 Dec 2009 - 11 Dec 2009)
  10. Development of chimeric Japanese encephalitis - Nipah candidate RNA vaccine construct using long range polymerase chain reaction assembly., The 3rd Regional Conference On Molecular Medicine, Institute for Research in Molecular Medicine (INFORMM) (International) (02 May 2009 - 04 May 2009)

ACADEMIC/PROF. SERVICES

Evaluation
  1. (2024) Virology Journal - Article Reviewer, International, (Reviewer)
  2. (2024) Virology Journal - Article Reviewer, International, (Reviewer)
  3. (2024) Scientific Reports - Article Reviewer, International, (Reviewer)
  4. (2024) Bmc Infectious Diseases - Article Reviewer, International, (Reviewer)
  5. (2024) Bmc Veterinary Research - Article Reviewer, International, (Reviewer)
  6. (2023) Bmc Research Notes - Article Reviewer, International, (Reviewer)
  7. (2023) Scientific Reports - Article Reviewer, International, (Reviewer)
Contribution to external organisation
  1. (2024) Bandung Medical Journal - Editorial Board Member, International, (Expert Advisor)
  2. (2023) Pejabat Kesihatan Lembah Pantai - Sukarelawan Mychampion, National, (Resource Person)
Contribution to event
  1. (2022) Moderator for Online Webinar Titled "Rabies: Apa Yang Saya Patut Tahu?" Organized by Malaysian Society of Parasitology and Tropical Medicine (Mstpm), National, (Panelist/Moderator)

PATENT/IPR

  • Antiviral activity of baicalein againts Japanese encephalitis virus
    Patent (International)
  • Antiviral activity of quercetin againts Japanese encephalitis virus
    Patent (International)

SOCIAL RESPONSIBILITY ACTIVITIES

  • Knowledge and Technology Transfer of Mosquito Identification Technique and Diy Ovitrap With Combi Squad At Jalan Ciku, Taman Kota Masai, Pasir Gudang, Johor, (19 Sep 2019 - 20 Sep 2019)
  • Community Empowerment - Field Trial of An Attracticidal Mosquito Coil With Combi Squad At Jalan Ciku, Taman Kota Masai, Pasir Gudang, Johor, (01 Jan 2017 - 30 Jun 2018)
  • Knowledge and Technology Transfer of Spokojan to Orang Asli Community in Kampung Dusun Kubur, Jelebu, Negeri Sembilan, (01 Aug 2013 - 31 Jul 2015)